In Vitro And In Vivo Anti-inflammatory And Hepatoprotector Effects Of Algerian Echium Plantagineum And Brassica Rapa Extracts

Résumé: This study aims to explore the phenolic composition of aqueous(Aq E)and methanolic(Met E)extracts of Algerian Echium plantagineum aerial part and evaluate their antioxidant capacity. Methods:Total phenolics, flavonoids and tannins contents were determined by spectrophotometric methods using Folin-Ciocalteu, aluminium chloride and hemoglobin precipitation assays,respectively.The antioxidant activities were evaluated using several in vitro tests including reactive oxygen species(ABTS.,OH.,O2.and H2O2)scavenging assays,ferrous ions chelating test, reducing power and anti-hemolytic assays.Results: The phytochemical study revealed a predominance of tannins, total polyphenols and flavonoids in both extracts.Aq E showed more efficiency to scavenge ABTS.,O2.and OH. radicals and to chelate ferrous ions. However,Met E was more efficient(IC50=193.87μg/mL)in scavenging H2O2 than Aq E (IC50 = 262.46μg/mL) . In the same way,Met E inhibited AAPH induced hemolysis(HT50=179.79±5.82 min)more than Aq E(HT50=161.62±5.87 min).On the other hand,both Met E and Aq E Exhibited very high reducing power (IC50= 117μg/mL and 128μg/mL, respectively). Conclusion: This study shows that Echium plantagineum extracts possess strong antioxidant activity,which is probably due to their phenolic composition.Therefore, this plant can constitute a promising source of natural antioxidants.

Publié dans la revue: