Effect Of Extraction Mode On Phenolics Contents And Biological Activities Of Two Species Of Thyme Extracts.

Résumé: Description of the subject: The extraction of bioactive compounds from plants was influenced by several parameters like the mode of extraction and solvents used. Our interest in this report turns up from these parameters. Object: The areal part of Thymus Capitatus and Thymus fontanesii, were subjected to a different mode of extractions, then the phenolics compounds were measured after that the biological activities were evaluated. Methodology: The decoction, infusion, hydro-alcoholic and acidified hydro-alcoholic mode and solvents were used for the extraction. The quantification of phenolics compounds was done by spectro-photometric tests. Antioxidant activities were done by scavenging of DPPH, reducing power test and total antioxidant capacity assay, furthermore, the antimicrobial activities were realized by disc diffusion and agar dilution methods. Results: For both species, total phenolics and flavonoid contents were higher for the hydro-methanolic extracts. Concerning the antioxidant activities, the decoction of T. capitatus was the most active extract in DPPH and reducing power tests, but at the total antioxidant capacity, T. fontanesii acidified hydroalcoholic extract, have given the higher content. In the end, the hydroalcoholic extract of both species was more active than the aqueous extracts. Conclusion: The data from this study support the idea that each solvent and mode of extraction chosen has its characteristic effects on yield of extraction, polyphenol quantities and biological activities. Keywords: T.capitatus, T.fontanesii, mode of extraction, antioxidant antimicrobial activities.

Publié dans la revue: AGROBIOLOGIA