Biochar-mediated Remediation Impacts On Nitrogen Cycling Bacteria And Ammonia Monooxygenase Activity In Crude Oil Polluted Soil
Résumé: Thisstudy adopted an ecosystem services approach to pollution management by investigating the impact of biochar-mediated remediation on soil nitrogen, abundance of nitrogen cycling bacteria and the activity of ammonia monooxygenase (AMO) enzyme in petroleum-polluted soil using two biochar types applied at two treatment levels with monitoring over 15 weeks. The corn cob-derived biochar (CDB), generally, had a stronger restorative effect on soil ammonium nitrogen,nitrate and total organic nitrogenconcentrations than the bone-derived biochar (BDB). Both biochar types had a more robust impact on restoration of Nitrosomonas, Nitrobacterand Azotobacterabundance (with the re-establishment of pre-pollution levels) than on Rhizobiumand Pseudomonas aeruginosa. Biochar amendment restored the activity of AMO enzyme in the soil by week 15. The CDB (72.4% –73.7%) showed more effective total petroleum hydrocarbon (TPH) elimination capacity than the BDB (51.1% –57.7%).Biochar amendments exhibited great potential for restoration of nitrogen cycling while facilitating remediation of petroleum-polluted soils.
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