دراسة المستخلصات العضوية والمائية لنبات Moltkiaciliata وتطبيقاتها في الفاعلية البيولوجية وتثبيط التآكل المائي
2021
Thèse de Doctorat
Mathématiques

Université Kasdi Merbah - Ouergla

س
سمية, شيحي

Résumé: This study aims to determine the anti-oxidant and anti-bacterial activity of the organic extracts of Moltkiaciliata obtained using the two systems (acetone / water) and (methanol / water) (80/20), as well as the estimation of the anti-corrosion activity of the aqueous extract.The results of thetests phytochemical showed its richness in most of the active compounds, while the extracts of ethyl acetate and butanol gave the highest extraction yield in both extraction systems, so the study summarized it.The results of chromatographic separation proved using several mobile phases, the best of which was Méthanol/Chloroforme (3/1). The studied extracts were rich in flavonoid compounds, and using HPLC and CCM technology, they were proven to contain many reference compounds and in different quantities.Quantitative estimation of phenolic compounds and flavonoids showed their presence in significant quantities. In comparison between the two systems, we find that the extracts obtained from the system (acetone / water) contain the largest quantities of phenolic compounds and flavonoids compared to the extracts obtained from the system (methanol / water).The antioxidant activity was estimated by several methods (DPPH, RF, PPM, ABTS, cyclic voltammetry method), where all the extracts showed significant antioxidant activity, and the differences in effectiveness among them can be explained by the differences in the chemical content.The antibacterial activity was estimated on three bacterial strains:Staphylococcus aures, Escherichia coli and Pseudomonasaeruginosa using the disk diffusion method . The results showed that it had a limited inhibitory activity against bacterial strains.Determination of the inhibitory activity of the aqueous extract on the corrosion of carbon steel XC70 in acidic medium (HCl, 0.IN) at different volumetric concentrations ( 2% to 10%), using several methods (gravimetric method (loss in mass),polaraisation curves and electrochemical impedance spectroscopy), where the results showed that the inhibition efficiency increases with increasing concentration of the extract with maximum inhibition percentage 76.26% at 8% concentration, the effect of temperature on corrosion speed was studied and calculation of the activation energy, enthalpy, Entropy, free energy and heat of adsorption, showed that the adsorption of the studied inhibitor is physical and exhibits a mixed behavior and follows the Langmuir adsorption model.

Mots-clès:

الفاعلية المضادة للأكسدة
الفاعلية المضادة للبكتيريا
استخلاص
التآكل
moltkiaciliata
activité antioxydante
activité antibactérienne
extraction
corrosion
antioxidant activity
antibacterial activity
extraction
corrosion inhibition
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