Isolation And Characterization Of A Polygalacturonase Activity In Five Local Strains Of Microscopic Fungi

Résumé: Five strains of microscopic fungi belonging to genus: Mucor. Aspergillus. Penicillium, Alternaria and Nigrospora were isolated from a soil of El kala region (north east Algeria). The study of the pectolytic activity showed that the five strains were active on the pectic substrat used as the only carbon source and they have secrete polygalacturonases in the liquid medium. These enzymes are inducible, have an acting pH varying between .f and 5.5 and are activated by monovalent ions (NaCI) situated between 5 and lOnuH. Polygalacturonases act on the pectic acid as a preferential substrat and galacturonic acid is the final product of degradation. Moreover. the detailed study of Mucor sp. strain has permitted 10 isolate and to partially purify an endopolygalacturonase EC (3.2.1.15).

Publié dans la revue: Synthèse