Extraction, Identification And Determination Of The Biological Activities Of Certain Active Extracts Of Wild Plants (peganum Harmala, Artemisia Campestris, Opuntia, Picris Hieracioides) In The Region Of Djelfa - Algeria.
2023
Mémoire de Master
Biologie Et Sciences De La Nature Et De La Vie

Université Ziane Achour - Djelfa

B
BOUCHIBA Yahia/HARFOUCHE Bouthina Rabab, HAMIDAT Rym Nour el Houda

Résumé: There are numerous medicinal plants in the region of djelfa in Algeria, including Peganum harmala, Artemisia campestris, Opuntia, and Picris hieracioides. Traditional medicine employs these plants due to their medicinal properties. Algeria's diverse plant flora contains numerous medicinal plants, such as Peganum harmala, Artemisia campestris, Opuntia, and Picris hieracioides. Traditional medicine has utilized these plants for their therapeutic potential. High yields of organic extracts were obtained from Peganum harmala leaf extract and Artemisia campestris roots. Utilizing the Folin-Ciocalteu method, the total phenolic content was determined. The concentration was highest in the aerial portion of Artemisia campestris (435.86 mg EAC/g of extract), followed by the root portion (323.69 mg EAC/g of extract). The AlCl3 method was used to determine flavonoid content, with assay results ranging from 95.70 to 2.60 mg EQ/g of extract. The leaf extract of Picric hieracioides exhibited the highest antiradical activity at 45.07%, surpassing all other extracts. Overall, however, the antioxidant capacity was only moderate. Using disk diffusion assays, antimicrobial activity was evaluated against six bacterial strains. The extracts showed sensitivity against all tested microorganisms, but Opuntia extract did not show any effect on Streptococcus pneumonia, Pseudomonas aeruginosa, or Staphylococcus aureus.

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