Effet De L'addition De L'urine De Chamelle Sur Les Activités Biologiques Des Caséines Isolées Du Lait Camelin À Différents Stades De Lactation
Résumé: This study was designed to evaluate the biological activities of camel caseins from different stages of lactation (alone or mixed with urine) hydrolyzed by digestive enzymes. Milk and urine were collected between March and April 2023 during the different lactation stages (2 days, 3 months, 6 months, 9 months and 12 months). After casein isolation, the action of three enzyme preparations (pepsin, presure and tryppsin) on caseins alone and mixed with urine was studied. The antioxidant activity of the ABTS test and the inhibition of alpha amylase of the various hydrolysates obtained were evaluated. The results showed that the hydrolysate of caseins alone at the 3-month stage after two hours of digestion had the best ABTS inhibitory capacity (IC50=0.19±0.00 mg/ml) compared with the other hydrolysates. This activity remains low in comparison with the standard used, tyrosine (IC50= 87.33± 0.00 µg/ml). With regard to anti-alpha amylase activity, the mixed hydrolysate (caseins+urines) at the 12-month stage obtained after 2 hours of digestion has the highest inhibitory power compared with other hydrolysates with IC50=0.32±0.03 mg/ml, but it is lower than that of the standard used, acarbose which has IC50=18.02±0.00 µg/ml.
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