Phytochemical Study And Biological Activity Of Different Extract From Flowers Of Parasitic Plant Cistanche Tinctoria (desf.) Beck.

Résumé: In order to evaluate the natural products of the desert plants, we conducted this work which is based on study the differences in amount of phenolic compounds and estimate the antioxidant activity of the flowers of the parasitic plant Cistanche tinctoria (Desf.) Beck (Orobanchaceae) growing in Oued-Souf region. After the extraction processes, the yield of the three extracts (Crude, Flavonoids phase Ethyl acetate and Tannins) was estimated with a variance in its values, the highest value recorded in the crude extract (11.26 %), however the tannin extract got the lowest value (0.505%). The results of the estimation of phenolic compounds showed that polyphenols and flavonoids were estimated by 167.74 ± 24.36 mg EAG/g Extract and 26.76 ± 2.69 mg EQu/g Extract, respectively. The antioxidant activity was performed by three tests, the results of the first one which is the DPPH• test, showed that tannin extract had the best inertial capacity than the other extracts (IC50= 8.12 μg/ml), however the results of Hemolysis test showed that the Flavonoid extract got the lowest disintegration (22.47%) between the extracts. The third test was the Reducing power assay and its results showed that the tannin extract had the highest reducing power between the extracts of Cistanche tinctoria (Desf). Beck. The three tests were applied using the Ascorbic acid as a standard compound. The qualitative analysis of the extract using High Performance Liquid Chromatography (HPLC) showed the differences of concentrations and types of some phenolic compounds in the crude extract of Cistanche tinctoria (Desf). Beck, such as: Chlorogenic acid (1.152 μg /mg Extract) and Naringin (27.456 μg /mg Extract).

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