Détection Des Résidus D'antibiotique Dans Une Série Des Tissus De Poissons D'aquaculture Par Une Validation Des Différentes Méthodes De Dépistages.

Résumé: The practice of antibiotic use in fish production and the presence of antibiotic residues have been studied by several researchers. Three scientific articles were selected to study the presence of these in farmed fish. The first article is presented under the title of antibiotic use and oxytetracycline residues in African catfish (Nigeria). The results showed that the majority of respondents (95%) frequently administered drugs (in water and feed) to their fish without veterinary prescription and without respecting the withdrawal period. The Commission established a maximum residue limit of 600 and 200 μg/kg respectively. This implied that a significant amount of fish presented risks related to antibiotic residues and food safety. The second article shows a determination of amoxicillin in catfish and salmon tissues by liquid chromatography with pre-column derivatization of formaldehyde. Mean recoveries of amoxicillin spiked at 2.5-20 ppb were >80% for catfish and >75% for salmon muscle tissue, with coefficients of variation of <6%. The limits of detection (LOD) and quantification (LOQ) for catfish tissue and salmon muscle tissue were 0.5-1.2 ppb and 0.8-2.0 ppb respectively. The third article investigates the primary validation of Charm II tests for the detection of antimicrobial residues in a series of aquaculture fish. The results of this study demonstrate the suitability of the Charm II technique as a rapid screening tool for the detection of antimicrobial residues in a variety of fish species at maximum residue limits (MRLs) set in EU guidelines.

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